high binding 96 well plates Search Results


96 well protein binding plates  (Revvity)
91
Revvity 96 well protein binding plates
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
96 Well Protein Binding Plates, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
96 well protein binding plates - by Bioz Stars, 2026-04
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santa cruz biotechnology cat  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology santa cruz biotechnology cat
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
Santa Cruz Biotechnology Cat, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
santa cruz biotechnology cat - by Bioz Stars, 2026-04
93/100 stars
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elisa plates  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology elisa plates
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
Elisa Plates, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elisa plates/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
elisa plates - by Bioz Stars, 2026-04
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high binding 384  (Santa Cruz Biotechnology)
91
Santa Cruz Biotechnology high binding 384
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
High Binding 384, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/high binding 384/product/Santa Cruz Biotechnology
Average 91 stars, based on 1 article reviews
high binding 384 - by Bioz Stars, 2026-04
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high-binding meso scale discovery 96-well plates  (Meso Scale Diagnostics LLC)
90
Meso Scale Diagnostics LLC high-binding meso scale discovery 96-well plates
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
High Binding Meso Scale Discovery 96 Well Plates, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/high-binding meso scale discovery 96-well plates/product/Meso Scale Diagnostics LLC
Average 90 stars, based on 1 article reviews
high-binding meso scale discovery 96-well plates - by Bioz Stars, 2026-04
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half-area 3690 eia plates  (Corning Life Sciences)
90
Corning Life Sciences half-area 3690 eia plates
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
Half Area 3690 Eia Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/half-area 3690 eia plates/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
half-area 3690 eia plates - by Bioz Stars, 2026-04
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high-binding 96-well immulon assay plates  (Fisher Scientific)
90
Fisher Scientific high-binding 96-well immulon assay plates
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
High Binding 96 Well Immulon Assay Plates, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/high-binding 96-well immulon assay plates/product/Fisher Scientific
Average 90 stars, based on 1 article reviews
high-binding 96-well immulon assay plates - by Bioz Stars, 2026-04
90/100 stars
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high bind plates l15xb-3  (Meso Scale Diagnostics LLC)
90
Meso Scale Diagnostics LLC high bind plates l15xb-3
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
High Bind Plates L15xb 3, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/high bind plates l15xb-3/product/Meso Scale Diagnostics LLC
Average 90 stars, based on 1 article reviews
high bind plates l15xb-3 - by Bioz Stars, 2026-04
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96-well half-area, low-binding, clear-bottom, and peg-coating plate  (Corning Life Sciences)
90
Corning Life Sciences 96-well half-area, low-binding, clear-bottom, and peg-coating plate
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
96 Well Half Area, Low Binding, Clear Bottom, And Peg Coating Plate, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/96-well half-area, low-binding, clear-bottom, and peg-coating plate/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
96-well half-area, low-binding, clear-bottom, and peg-coating plate - by Bioz Stars, 2026-04
90/100 stars
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white corning costar high binding polystyrene 96-well plates  (Fisher Scientific)
90
Fisher Scientific white corning costar high binding polystyrene 96-well plates
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
White Corning Costar High Binding Polystyrene 96 Well Plates, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
white corning costar high binding polystyrene 96-well plates - by Bioz Stars, 2026-04
90/100 stars
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high-binding, flat-bottom, 96-well plates  (HiMedia Laboratories)
90
HiMedia Laboratories high-binding, flat-bottom, 96-well plates
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
High Binding, Flat Bottom, 96 Well Plates, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/high-binding, flat-bottom, 96-well plates/product/HiMedia Laboratories
Average 90 stars, based on 1 article reviews
high-binding, flat-bottom, 96-well plates - by Bioz Stars, 2026-04
90/100 stars
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96-well ultra-low binding u-shaped bottom culture plate corning #07-202-463  (Corning Life Sciences)
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Corning Life Sciences 96-well ultra-low binding u-shaped bottom culture plate corning #07-202-463
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
96 Well Ultra Low Binding U Shaped Bottom Culture Plate Corning #07 202 463, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/96-well ultra-low binding u-shaped bottom culture plate corning #07-202-463/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
96-well ultra-low binding u-shaped bottom culture plate corning #07-202-463 - by Bioz Stars, 2026-04
90/100 stars
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HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to 96-well plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.

Journal: Journal of Virology

Article Title: The High Content of Fructose in Human Semen Competitively Inhibits Broad and Potent Antivirals That Target High-Mannose Glycans

doi: 10.1128/JVI.01749-19

Figure Lengend Snippet: HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to 96-well plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.

Article Snippet: To measure infection in synthetic or human SP, viruses suspended in PBS were attached to 96-well protein-binding plates (PerkinElmer) by spinoculation at 2,000 × g for 2 h at 10°C.

Techniques: Infection, Inhibition, Incubation, Cell Culture, ATP Assay, Saline, Virus, Two Tailed Test